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Image Search Results
Journal: PLoS ONE
Article Title: The In Vitro Effect of Acidic-Pepsin on Nuclear Factor KappaB Activation and Its Related Oncogenic Effect on Normal Human Hypopharyngeal Cells
doi: 10.1371/journal.pone.0168269
Figure Lengend Snippet: Luciferase assay demonstrates the effect of pepsin in NF-κB transcriptional activity in (A) HHK and (B) HHPC. Acidic-pepsin (pH 4.0) does not induce NF-κB transcriptional activity in treated human normal hypopharyngeal cells. Columns represent NF-κB relative transcriptional activity (NF-κB responsive luciferase reporter/control luciferase reporter). 3kB-ConA-luc: NF-κB responsive luciferase reporter; ConA-luc: control luciferase reporter; 3kB-ConA-luc/ConA-luc: NF-κB responsive luciferase reporter/control luciferase reporter. CA: Acid-Control; PA: Acidic-Pepsin; CW: Weakly Acidic-Control; PW: Weakly Acidic-Pepsin; CN: Neutral-Control; PN: Neutral-Pepsin; PI: Inactivated-Pepsin.
Article Snippet: The HHPC were plated in non-coated flasks and were grown in
Techniques: Luciferase, Activity Assay
Journal: Molecular Therapy. Nucleic Acids
Article Title: MiR-449a antagonizes EMT through IL-6-mediated trans -signaling in laryngeal squamous cancer
doi: 10.1016/j.omtn.2024.102140
Figure Lengend Snippet: Expression profile of miR-449a in LSCC cell lines (A) Relative basal miR-449a expression levels (mean ± SD) in FaDu, UPCI:SCC152,HNO-210 cell lines vs. human hypopharyngeal normal cell. (B) miR-449a relative expression levels in HNO-210 cells. (C) miR-449a relative expression levels in FaDu cells. Each data point was obtained in triplicate and data are shown as mean ± SD (Student’s t test) ∗p < 0.05, ∗∗∗p < 0.001.
Article Snippet: The HNO-210 cell line was grown in DMEM (MICROGEM DMEM Cat# AL066A), while the Human hypopharyngeal normal primary cell culture (CELPROGEN, Cat# 36074-04) cells were grown in human
Techniques: Expressing